HIV drug resistance to protease inhibitors?!
Question:
HIV drug resistance to protease inhibitors?
i am trying to develop a protocol to sequence HIVDR genes. i know that for the RT gene, codons 1-240 have been shown to be most involved in HIVDR . which codons in the Pr gene are most involved in HIVDR so that i don't have to sequence the entire gene?What primers would you recommend i use to amplify this region?
i would appreciate as much detail as possible.
thankyou.
Answers:
Common point mutations are at 10, 20, 24, 30, 32, 33, 36, 46, 47, 48, 50, 53, 54, 63, 71, 73, 77, 82, 84, 88, 90 and 93.
Resistance to protease inhibitors is complex, and usually depends on the accumulation of several different mutations, one after the other. There is often an interplay between mutations that confer resistance but reduce the ability to replicate, and those which increase the ability to replicate without further adding resistance.
Different PIs are likely to select for different mutations.There are also a number of single mutations, such as at 82, 84 and 90 which are regarded as mutations which confer resistance to all PIs.
See this page for more detail of other specific mutations:
http://www.aidsmap.com/cms1032055.asp...
No idea about the primers, though. I failed first year biochemistry and haven't been back. Got a job rounding up sheep, which I really enjoy.
You are asking a very technical question. My guess is that a satisfactory answer is more likely to come from reading primary sources such as original peer reviewed research in genetics, virology and pharmacology, rather than secondary sources like my link or very, very tertiary sources like Yahoo Answers.
'Scuse me, gotta go. I've got a wether that keeps trying to break out.