Whic one can ferment agar? or blood agar..?!
Question: Whic one can ferment agar!? or blood agar!.!.!?
staphylococcus epidermidis or staphylococcus aureus!?
one will give pink while one will give yellow colour in agar!?
help!Www@Answer-Health@Com
one will give pink while one will give yellow colour in agar!?
help!Www@Answer-Health@Com
Answers:
To ferment is aureus!.
Salt agar 7-10% ability to ferment, indicator media containing phenol red turns staphylococcus aureus colonies to YELLOW!.
When growing staph's on MacConkey's agar the colonies are PINK due to the neutral red indicator (pink in acid, colorless in alkaline!.)Www@Answer-Health@Com
Salt agar 7-10% ability to ferment, indicator media containing phenol red turns staphylococcus aureus colonies to YELLOW!.
When growing staph's on MacConkey's agar the colonies are PINK due to the neutral red indicator (pink in acid, colorless in alkaline!.)Www@Answer-Health@Com
On blood agar staph!. epidermidis will be very tiny white opaque colonies that have no zones of heamolysis - they will not break down the hame in the blood agar!. staph!. aureus will be larger colonies ranging from a pale yellow to an orange - depending on the strain!. staph aureus colonies will present a ring of beta haemolysis - a clear zone around the colonies, which shows that they do break down hame proteins in blood agar, which means they will do that in the body!. sometimes, the zone of haemolysis may not show, or be very faint, so more testing must be done!.
On MaConkey agar it is different, as you are testing to see whether a certain bacteria can ferment lactose!. MacConkey agar is usually tested to provide a diagnosis whether the bacteria is enteric (grows/lives in the gut) and gram negative!. this agar contains bile salts which will promote growth of gram negative bacteria (however it is possible it will include certain species of staphylococcus) and crystal violet which will inhibit growth of some gram positive bacteria!.
On a MacConkey plate that does not have crystal violet or salt, the staph aureus colonies will grow as pale pink, but on regular MacConkey plates that do conatin crystal violet and salt, there will be no staph!. aureus growth and also no staph!. epidermidis growth!.
On a MacConkey agar plate, bacteria that ferment lactose will show as pink as often mucoid appearance such as e-coli!. Colonies that appear yellow or transparent are not lactose fermenters!. These are bacteria such as pseudomonas aeruginosa and staph
I hope this has helped answer your question!.
Good luck :)
Www@Answer-Health@Com
On MaConkey agar it is different, as you are testing to see whether a certain bacteria can ferment lactose!. MacConkey agar is usually tested to provide a diagnosis whether the bacteria is enteric (grows/lives in the gut) and gram negative!. this agar contains bile salts which will promote growth of gram negative bacteria (however it is possible it will include certain species of staphylococcus) and crystal violet which will inhibit growth of some gram positive bacteria!.
On a MacConkey plate that does not have crystal violet or salt, the staph aureus colonies will grow as pale pink, but on regular MacConkey plates that do conatin crystal violet and salt, there will be no staph!. aureus growth and also no staph!. epidermidis growth!.
On a MacConkey agar plate, bacteria that ferment lactose will show as pink as often mucoid appearance such as e-coli!. Colonies that appear yellow or transparent are not lactose fermenters!. These are bacteria such as pseudomonas aeruginosa and staph
I hope this has helped answer your question!.
Good luck :)
Www@Answer-Health@Com